Comparison Between Protective Antibody Response And Guinea...

Comparison of protective Antibody response in guinea pigs immunized with Wild type (Wt) BoHV-1 and ΔgE BoHV-1 mutant virus vaccine L.R.ANANTHAKRISHNA1*, MOHINI SAINI2, PRAVEEN K GUPTA3 and RIZWANA TABASSUM4 1Asst.Professor, 2, 3 Principal Scientist, 3Research Associate 1Department of Veterinary Physiology and Biochemistry; Karnataka Veterinary, Animal Fisheries Sciences University; Veterinary College, Vinobanagar, Shimoga- 577 204, India, 2Division of Biochemistry, 3 4Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, Uttar Pradesh, India *1Email: drananthalr639@gmail.com contact: 9535205024 ABSTRACT Vaccination has been one of the most important interventions in disease prevention, control and eradication. The impact of vaccination largely depends on type of immune response and the extent of protective antibodies that would neutralize the infectious pathogen in subsequent exposure. BoHV-1 causes several clinical conditions in cattle including rhinotracheitis, vaginitis, balanoposthitis, abortion, conjunctivitis, and enteritis which impact a severe economic loss to dairy industry. When the ΔgE BoHV-1 mutant virus was intended to be used as vaccine one of the major immunological parameters such as virus neutralizing antibodies to protect animals against infected virus and/or immunized virus. To determine the suitability of a vaccine, in this study we performed in vitro test method to compare the level of